Analysis of genetic diversity of E.coli bacteria isolates from UTI after exposure to some biological effects by using RAPD technique

Abstract

Background and Objective: Escherichia coli  bacteria are one of the most important bacterial races causing urinary tract infection, which is responsible for 90% of the causes of urinary tract infection and originated in the human intestine, this is Gram- Negative bacteria and facultative aerobic that moves by peripheral flagella and belongs to the bacteria of the Enterobacteriaceae.

Materials and methods: The bacteria were isolated from UTI, after they were diagnosed using light microscope and conducting biochemical tests, then exposed to the antibiotic Ciprofloxacin, trimethoprim, and alcoholic extract of Punica granatum and Trigonella foenum graicum. genomic DNA was extracted for all samples and Random amplified polymorphic DNA - Polymerase chain reaction (RAPD-PCR) marker was carried out using five random primers.  

Results: The results of RAPD-PCR profiles shown that exposed to antibiotics (Ciprofloxacin and Trimethoprim) and alcoholic extracts (Punica granatum and Trigonella foenum graicum) lead to the disappearance or appearance new bands compared with non-exposed samples, and the highest rate of polymorphism for all each treatment and primers in sample 5 was 102.27% where the ratio % GTS for all treatment and primers is 6.04% in the same sample.

Conclusion: All treatments caused genetic changes in the DNA of E.coli bacteria cells especially the Punica granatum which gave the highest effect than the rest of the treatments, this indicates its efficiency in treating bacterial infections.