Tikrit Journal of Pure Science

Estimation of the concentration of some biochemical parameters and bacterial profile in patients with periodontitis and its relationship with cardiovascular disease

Duaa raad Saleh — 2024-10-25

Background: Concerning the widespread occurrence of hyperlipidemia, a significant risk factor for cardiovascular disease, and unclear findings on the connection between periodontal disease and the levels of lipids in the blood. Aim of the study: This study aimed to isolation different bacterial type from periodontitis patients and evaluated level of lipid profile in the same patients

Material and methods: The study included a collection age group of 90 samples swaps (patient and control), 60 samples from patients with periodontitis attending outpatient clinics and Balad General Hospitalthe sample were collected for different ages between 15-55 years old and both sexes, from july 2023to December 2024. The sample was taken using a sterile cotton swab (transport cotton swab). The blood samples were taking from their arms from (cubital vien), 5cc of venous blood taken from patients & control healthy individual, Samples were cultured directly using the plotting method on culture media suitable for bacterial growth, which included blood agar, MacConkey agar, mannitol salt agar, MSB agar, and chromo agar. The plates were incubated upside down at 37°C for 18–24 days. The bacterial isolates were identified based on physiological tests and biochemical tests.

Result: The present study’s result showed About (60%) showed positive bacterial growth which were Prophyromonas gingivalis (30%), Staph. aureus (16.7%), Strep. pneumonia (11.7%), Staph. epidermidis (10%), Strep. pyogenes, Strep. Viridans, Strep. mutans (8.3% %) for them, and (6.7%) for Enterococcus fecalis that was diagnosed based on biochemical tests, and the diagnosis of some bacterial species was confirmed with the VITEK device., whereas (40%) showed no growth, And there were a high significant differences between the periodontitis patient compared with control group in mean level of cholesterol, HDL and LDL which scored (151.1±42.7, 139.9±41.4, 184.9±25.4) respectively in patient compared with controls (80.0±16.8, 46.2±14.6, 99.1±27.1). While Triglyceride and VLDL showed no differences between studied groups. Conclusion: This study concluded increase cholesterol and low-density lipoprotein in periodontitis patient, While no differences in Triglyceride and VLDL level

Firas Tariq Mahmood, Firas faris Evaluation N-terminal pro-B-type natriuretic peptide and other biochemical parameters in heart failure with or without chronic kidney disease in Kirkuk city

Firas Tariq — 2024-10-25

Backgrounds: Individuals who are diagnosed with chronic kidney disease (CKD) are at a significantly increased risk of developing cardiovascular (CV) disease. Aim of the study: This study evaluated N-terminal pro-B-type natriuretic peptide, urea, creatinin, and albumin in heart failure with or without chronic kidney disease. Materials and methods: The study was done at Amal Dialysis Centre, General Kirkuk Hospital Lab and Azadi Teaching Hospital Lab. The age range of the participants was between 18-68 years old. The study was conducted from January to Augest 2023. The current study examined 90 participants that divided in to four groups as: G1=Kidney failure patients (n:23), G2=Heart failure patients (n:22), G3=Heart and kidney failure (n:23), and G4=apparently healthy normal individuals. Results: This study show highly significant increase NT Pro- BNP in G1, G2 and G3(123.88±22.73, 107.86± 20.15, 134.85± 26.05), as compared with G4 (67.19±18.44) at p-value<0.0008. While highly decrease albumin in G1,G2 and G3(4.318±0.486, 4.028±0.991, 5.500± 0.652), as compared with G4 (7.028± 0.991) at p-value <0.01Conclusion: This study concluded increase in NTpro Pro- BNP, urea and creatinin in all three groups as compared with control group and decrease in albumin level.

Younis Shehab Ahmed 1 andBashar Evaluating the efficiency of oil waste treatment plant in AL- Qayyarah Refinery - Iraq

younis shahab ahmad — 2024-10-25

The study included an evaluation of the efficiency of AL-Qayyarah refinery oil waste treatment plant. For this purpose, monthly samples were collected for a period of six months from October 2023 until March 2024, the first station (before treatment) represents the water coming out of the crude oil filter units. As for the second station (after treatment) it is located in the refinery waste treatment unit, the site represents the treated water. many physical and chemical tests were conducted on water, they ranged of temperature values before the treatment ranged between (17-33) compared to its value after the treatment (15-31), and the electrical conductivity values before the treatment ranged between (295-405)µS/cm, while after the treatment it was (349-471)µS/cm. As for total dissolved solids, their average before the treatment was(182.5)mg/L, while their average after the treatment was(194.6)mg/L as for salinity, its values ranged between(0.7-0.75) mg/L before the treatment and between (0.6 -0.8) mg/L after the treatment ,pH value before the treatment was between)6.12- 7.91( compared to its value after the treatment as it ranged between (6.81-7.95), while the values of the chemical requirement for oxygen before the treatment were (85108)mg/L, Their concentrations values after the treatment amounted to(73_125) mg/L, and polycyclic aromatic hydrocarbons (PAHs) (73.46-155.17) mg/L before the treatment, and after the treatment it reached (35.53-52.53) mg/L, while oils and greases reached values that ranged between (26.16-165.68) mg/L before the treatment compared to their results after the treatment which ranged between (94.15-23.18)mg/L. Finally, the total organic carbon had an average value before treatment (134.1) mg/L, while after treatment their average values reached (120.8) mg/L

Evaluation of Geh gene expression in Staphylococcus aureus isolated from acne patients

mohammed hussein — 2024-10-25

Acne vulgaris is a chronic skin disease that affects the hair follicle and sebaceous glands. Staphylococcus aureus colonizes human skin and causes infections ranging from local skin infections to systemic infections that may threaten human life due to its ability to produce many virulence factors and antibiotic resistance. The objective of this study is to measure the genetic expression of Geh gene responsible for producing the lipase enzyme from S. aureus isolated from acne patients. This study was conducted by collecting 130 swabs from acne patients at Tikrit Teaching Hospital. Using routine bacterial isolation and identification methods, 50 samples (38.4%) of the total samples were identified as S. aureus through its ability to grow in mannitol salt agar medium, the fermentation of mannitol sugar, and the change of the medium color to yellow. The Geh gene expression of ten S. aureus isolates from acne patients was examined using RT-PCR. The results showed that Geh gene was expressed in all isolates, with an expression ranging from 1.5 to 7.2, and that the mean folding of the Geh gene for ten samples was 4.2. This indicates the presence of high gene expression of the Geh gene in S. aureus isolates. Therefore, it is concluded that Lipase enzyme plays an important role in the pathogenesis of S. aureus in acne patients.

T Kinetic and thermodynamic study of the adsorption isotherm of methylene blue dye from aqueous solutions by activated carbon

Asraa Fawzy Mustafa Altikrite — 2024-10-25

The efficiency of activated carbon in wastewater as a powerful blue dye remover has been studied. In this work, activated carbon manufactured from Sidr leaves was used to adsorb methylene blue dye. The factors affecting adsorption were studied, such as the weight of the adsorbent, temperature, contact time, and initial concentration of the dye, and the optimal conditions for adsorption were determined according to the experimental results. It was evident from the experimental results obtained that the removal efficiency increases with the increase of both the weight of the adsorbent material and the contact time. It was observed that the adsorption process was possible, spontaneous, and exothermic in the temperature range (293-333 K), and this is what determined the thermodynamic variables ΔG°, ΔH°, and ΔS°. The Langmuir, Freundlich, and Timken isotherms were used to examine the experimental equilibrium data. To follow the adsorption process, two logical models were chosen, represented by the Elovage equation and diffusion.

Effect of Chloride Solution on Liquid Limit, and Plasticity Index of Soil in Selected Sites in Erbil /North Iraq

Nadhmia Najmaddin Majeed — 2024-10-25

This study is an attempt to investigate the effect of NaCl, CaCl₂, KCl, and NH₄Cl solutions with different concentrations (2.5%, 5%, 7.5%) on the liquid limit, plastic limit, and plasticity index of Sarbasty and Mamwastayan soil samples /Erbil Governorate. The soil samples' liquid and plastic limits were found by using the cone penetrometer method. The Results showed that the soil's liquid, plastic limits, and Plasticity index decreased with increasing salt solution concentration. Maximum reduction of liquid limit and plastic limit in Sarbasty was from 53.6 to 34.3 and in Mamwastayan from 35.9 to 27.2, both accomplished by adding a concentration of 7.5% of KCl and CaCl₂respectively. According to the Unified Soil Classification System, the Sarbasty soils were classified as high plasticity clay (CH), and Mamwastayan low plasticity clay (CL). After adding different concentrations of salt solution to the soil, the Sarbasty soil classification changed from CH to CL. Mineralogically these soils are composed of non-clay minerals of calcite and quartz and clay minerals consisting of Montmorillonite, Chlorite, swelling Chlorite, Kaolinite, Illite, Palygorskite, and Illite- Chlorite. Sarbasty soil was the most effective salt chloride solution than the Mamwastayan soil sample swelling.

Study The Effect of Doping Zinc Oxide Thin Films With (0.5 wt. %) Carbon Nanotubes by Vacuum Evaporation

ahmed mansoor — 2024-10-25

The study included the production of nano-thin films made of zinc oxide doped with carbon nanotubes (ZnO:CNTs) at a doping ratio of 0.5 wt%. The materials are applied onto glass substrates using the vacuum evaporation process. The structural characteristics of the thin films of undiluted and doped ZnO were assessed using X-ray diffraction (XRD). Additionally, the surface properties of the films were examined using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The optical characteristics of the thin films were analyzed and described using UV-Vis spectroscopy. The ZnO thin films exhibited crystalline formations. The thin films exhibited significant orientations along the (100), (002), and (101) crystallographic planes, indicating their hexagonal phase structures. The crystal size of ZnO thin films exhibited a range of 30.87 nm to 19.96 nm after the process of doping. The study findings demonstrate that the addition of carbon nanotubes leads to an increase in the absorption ratio. Zinc-doped carbon nanotube thin films has features that make them suitable for many applications, such as gas detectors and UV detectors.