Study of Genotoxic and Cytotoxic effects of Malathion in Japanese Quail

Abstract

The effect of Malathion was studied orally on Japanese quail males (Cotrunix Cotrunix Japonica). The Median lethal Dose LD50 of  malathion through 24 hours was found to be 163.6 mg / kg. Malathion was provided in doses of 75% 122.7 mg / kg b.wt., 50% 81.8 mg / kg b.wt., 25%40.9 mg/kg b.wt. LD50 of body weight plus cyclophosphamide 20 mg/kg b.wt as positive control and Corn oil as a negative control, The current study was carried out to detect the effects of malathion on cell cytotoxicity based on genetic cytotoxicity tests such as Micronuclei Test (MN for mature red blood cells in peripheral blood and immature blood cells in bone marrow for 18, 20 and 22 hours per treatment and Chromosomal Aberration (CA) test for immature red blood cells in bone marrow, And 24 hours for each treatment with subcutaneous injection of colchicine 0.5gm prior to 3-hour incubation. The results of the statistical analysis Suggested that a significant increase in P≤0.05of micronucleated mature red blood cells in peripheral blood and micronucleated immature blood cells in bone marrow for 18, 20 and 22 hours per treatment in the formation of micronuclei compared to negative control. The results of the study also Suggested that a significant increase in P≤ 0.05  on the induction of chromosomal Aberration  of immature red blood cells in bone marrow.