Isolation and purification of copeptin hormone from serum of patients with heart disease
DOI:
https://doi.org/10.25130/tjps.v20i3.1191Abstract
This study included isolation and purification of copepin hormone from heart disease patients serum using different biochemical techniques. The study showed two proteinous peak has been isolated by gel filtration (Sephadex G-50) from the precipitation by cold acetone, the results predicted that (peak B) has a high concentration of copepin . Furthermore, the purity of the isolated copeptin ( peak B) had been identified by SDS-poly acryl amide gel electrophoresis and the reverse phase high performance liquid chromatography in which results showed agood identity in retention time between the standard and the isolated copeptin (peak B) .
The approximate molecular weight of partially purified copeptin peak (B) was (4654±400 Dalton , ±4513400 Dalton) using gel filtration chromatography (Sephadex G-50) and (SDS-poly acryl amide gel electrophoresis) technique respectively .
The study of secondary structure (α-helix) to copeptin hormone by the (Circular Dichroesim) showed the percentage of the (α-helix) between the standard solution of copeptin and sample solution (peak B) was good match.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tikrit Journal of Pure Science
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
