Spectrophotometric determination of Folic acid (B9) by oxidative Coupling Reaction with 4-nitro-aniline
DOI:
https://doi.org/10.25130/tjps.v27i6.755Abstract
Development of an easy, inexpensive, fast and selective spectrophotometric method for the determination of Folic Acid (FA) by its reaction with 4-nitro aniline reagent, with good sensitivity, as an oxidative coupling method in the presence of Potassium permanganate solution, and it was found that the concentrations that were agreement with Beer-Lambert’s law at the wavelength of 374 nm ranged between 1- 21 µg/ml of folic acid solution, and a deviation from Beer’s law occurs at a concentration higher than 21 µg/mL, gave a linear coefficient and correlation coefficient its value is 0.9994, 0.9996, respectively, While the value of the molar absorption coefficient 12138.5 L/mol.cm and Sandel's sensitivity 0.0363 gm/cm2 with the limits of qualitative and quantitative detection 0.103 and 0.363 μg / ml-1, respectively. The proposed method was successfully applied for the determination of folic acid in its pharmaceutical preparations, from Erbil Company – Iraq. Trade name (folic acide).
Downloads
Published
How to Cite
License
Copyright (c) 2023 Tikrit Journal of Pure Science
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.
