isolates multi resistant to antibiotics and observed by Scanning electron microscopy

Abstract

One hundred fifty six samples collected included: inflammation of the urinary tract, swabs wounds and pus from patients coming to Rizgary Teaching Hospital and Rozhawa Hospital in Erbil city from March to September 2013, scrubbed and confirmed the diagnosis (42) strain which belongs to staphylococcus aureus and by (26.9%) based on cultural characteristics, microscopically features and biochemical tests in addition to the API Staph. These strains sensitivity to 12 types of antibiotics. It gave the species a high resistance against the Ampicillin (AM / 10μg) by 88% and resistant to Amoxicillin (AX / 25μg)by (81%) were less resistant to Nitrofurantoin (NF / 300μg), Ciprofloxacin (CIP / 5μg), Gentamicin (GM / 10μg ) by (31,26,21.4%) respectively Ten isolates were selected according to their pattern of the highest resistance as these showing multi-drug resistances and tested to specify their minimum inhibitory concentration (MIC) for the antibiotics and two types of Nanoparticles include Silver in different sizes (20, 90)nm and Zinc Oxide in different sizes (20, 30, 50~150)nm. The results showed that the MIC for Ag 20 nm was between (650-2600) μg/ml while Ag90nm was between (325 -2600) μg/ml and the MIC for ZnO20nm between (325-2600) μg/ml and MIC of ZnO 30, 50 ~ 150nm between (162.5-2600) μg/ml.  Synergism effect between the antibiotics and the Nanoparticles when they integrate increased their effect of Staphylococcus aureus. Morphological changes of bacteria found using light and scanning electron microscope (SEM) when treating with Nanoparticles. While there a pressure on the bacterial cell surface with losing of bacterial compound.