Bacteriological and Genetic study of some gram negative bacteria isolated from different infections

Abstract

There are 30 of  bacterial samples were isolated from 45 samples of different infections. These isolates were diagnosed microscopically and by using biochemical tests. ability of their resistance was tested against the following  antibiotics: (amoxicillin, tetracycline, cephalexin, nitrofurantoin, ciprofloxacin, clarithromycin, erythromycin, ampicillin, rifampin), and for detection the sites of  genes  encoding for  antibiotic resistance , bacterial conjugation experiments was done for some of bacterial isolates that contain plasmids. Results of bacterial conjugation has revealed that antibiotic resistance genes in isolates (P. aeruginosa (14), E.coli (4)) were present on DNA plasmid , not on chromosome. But the other isolates, we could not find conjugated  bacterial colonies for them which indicate that there DNA plasmids do not have auto transmission.