Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation
DOI:
https://doi.org/10.25130/tjps.v24i1.333Abstract
In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524 nm. This method was developed to determination of thiamine hydrochloride by bleaching the red colour (alizarine-sulphite product) at the optimum conditions established for the determination of sulphite. Beer’s law is obeyed in the concentration range (10-1000) μg/25ml (0.4-40) of sulphite and 10-1500, μg/25ml (0.4-60) for thiamine hydrochloride at the selected wavelength, the molar absorbtivity 2.6×104 l.mol-1.cm-1 for SO32- and 3.37×104 l.mol-1.cm-1 for thiamine hydrochloride, Sandell's index for sensitivity 0.00307 μg .cm-2 for sulphite and 0.0100 μg .cm-2 for B1, the precision (RSD) of the method is ±0.23- ± 0.51% sulphite and ±0.15- ±0.25 thiamine hydrochloride. Good recoveries of sulphite and thiamine hydrochloride from water samples and pharmaceutical formation are achieved using the proposed method.
Downloads
Published
How to Cite
License
Copyright (c) 2022 Tikrit Journal of Pure Science
This work is licensed under a Creative Commons Attribution 4.0 International License.
Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.