Determine the Ability of Bacterial Isolates from Urinary Tract Infections on L-Asparginase Production

Abstract

This study was conducted to isolate and identify the pathogenic bacterial species from urinary tract of patients that reviewers to Tikrit hospital education from tow gender and different age periods. It was also the determination of bacterial species isolated in their ability to production of the L-asparginase enzyme and assay the environmental conditions and the production media ingredients for high concentration of enzyme production. Results showed that 142 samples was positive in microbial isolation (91.64%) from the total study samples that were at 150 samples, and high isolation percentage was from female at 58% and from aged period between 15 to 75 years for two gender. It also found that the species of bacterial E.coli was appear as highest in isolation percentage followed by Citrobacter diversus, Enterobacter aerogenes and Klebsiella pneumonia which was at 54.2, 21.1, 17.6 and 7.04% respectively. The assay of bacterial isolates to L-asparginase produce illustrate that the E.coli species appear as the most production and the isolate no. 3 was the top one at 70.4 IU/ml of media. When the assay of environmental conditions and ingredients combination for media to access for higher production from enzyme, shows that the optimal conditions for production of E.coli-Iso.3 was in development at 35 º c, pH 7.0 for 24 hours with agitation at 150 rpm in the media consists of maltose, L-asparginase, NaCl and KH2PO4 at 10, 100, and 5.0 and 0.75 g/l respectively.