Estimation of 5`-nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes

Authors

  • Asra'a Ismail Yaseen Ahmed AL-Taii

DOI:

https://doi.org/10.25130/tjps.v20i2.1167

Abstract

This study was performed on (60) saliva specimens, including (30) diabetic patients in addition (30) healthy saliva specimens were taken from healthy subjects as control group. The results showed a significant increase in the activity of saliva 5`-nucleotidase (5`-NT) from diabetic patients compared to healthy subjects.

This study included a partial purification of 5`-NT as well, using (35%) (NH4)2SO4   for precipitation and gel filteration by (sephadex G-75 ) which gave single peak. Then 5-NT was partially  also purified by using Ion exchange chromatography ( DEAE-cellulos A-50), in which three isoenzymes were isolated with different degree of purity.

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Published

2023-02-09

How to Cite

Asra'a Ismail Yaseen Ahmed AL-Taii. (2023). Estimation of 5`-nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes. Tikrit Journal of Pure Science, 20(2), 105–109. https://doi.org/10.25130/tjps.v20i2.1167

Issue

Vol. 20 No. 2 (2015)

Section

Articles

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Copyright (c) 2023 Tikrit Journal of Pure Science

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