The effect of Borag officinalis infusion in histological structure of lung and some blood parametars in white male mice

Abstract

This study is designed to recognize the side effects of Borago officinalis infusion on the histological structure of  lung and some blood  parameters (WBCs, PLTs, Hb ,P.C.V ).

Ten Swiss white male mice were used,  they were   divided into two groups:

First group (G1): included five animals given a dose of 0.5ml of the physiological solution (Normal saline) by means of an oral-stomach pipe, for 10 days. It was considered to be the control group.

Second group (G2): included five animals given a dose of 0.5ml of Borago officinalis infusion by concentration %7 by means of an oral-stomach pipe for 10 days.

At the end of experiment all animals were killed and colleccted blood samples were collected gathered in  tubes containing EDTA  salt, and the Lungs were removed and preserved  in  formalin 10% .

The results showed the following:

1. A significant decrease (P≤0.05) occurred in the rate of (P.C.V) bulk volume percentage of blood and Hb concentration of the second   group(G2)  when compared with the animals of the control group . And there was a significant decrease (P≤0.05) in the average of cellular heamoglobin MCHC of the (G2) compared with control group animals.
2. There was a significant increase (P≤0.05) in the rate of blood platelets (PLT) and in the rate of the total amount of the white blood cells (WBC),in second group (G2 ) compared with the control group (G1).
3. Histological examination showed occurrence of degenerative changes in the histological structure of the mice lung Hyperplasia and infiltration of lymphocytes in the walls of the trachea and rupture the walls of the alveoli in some areas and increase the thickness of the walls in other areas .

This study concluded that infusion Borago officinalis concentration 7% revealed a negative effected on the histological structure of lung and some blood parameters .