Spectrophotometric Determination of para aminosalicylic acid in Serum Blood and Urin By Oxidative Coupling With 4-Amino Antipyrine and Study The Inhibition of Aldose Reductase Enzyme

Abstract

This work includes a new spectrophotometric method for the determination para aminosalicylic acid in aqueous medium based on the oxidation by  NaIO₄ and coupling with 4-Amino Antipyrine producing a red dye soluble in water  and of ג_max of 516 nm. The proposed method could be applied successfully for the determination of para aminosalicylic acid, with a molar absorptivity of 17568 L.ml ^-1. Cm ^-1 and the Value of Sandell's sensitivity 0.0120 μg.cm ^-2. The results showed good accuracy and compatibility with Value of the relative error (1.00- 3.65 +) % relative standard deviation of (0.008 to 0.1305)% depending on the level of concentration. The application of the proposed method Show that the Beer's law is obyed range of concentrations of 10-500 μg in a final volume of 25 ml. The new method  was used for estimation of para aminosalicylic acid in  Serum and urine, and study it's  effect to Aldose reductase  which partially purified from sera of patients with type II diabetic mellitus. The results indicate that reactivity perentaye were 101.395 in serum and  100.776 in urine, while the inhibition percentage for aldose reductase were increased with increasing of inhibitor concentration  (16-84)% .