Study Genotoxic and Cytotoxic effect of the fungicide Propamocarb- HCl in Albino mice

Abstract

The study was designed to evaluate genotoxicity and cytotoxicity of the fungicide probamocarb- HCl using  Swiss white  mice Mus musculus .

The evaluation of treatment effects with  the dosage 1355 and 2349 mg/kg .bw of the fungicide was depended on genomarkers (Micronuclei in bone- marrow cells, evaluation of DNA damage in liver and bone-marrow cells using comet assay).

The results showed significant linear increase at p≤0.05 between time of treatment and micronuclei which reached 420.40 ± 12.21 with replicate treatment of the dosage 1355 mg/kg.bw of the fungicide compared with 4.80 ± 2.10 for negative control and 592.60±5.83 with single dosage of 2493 mg/kg. bw of the fungicide. Change in the percentage of polychromatic erythrocytes also observed which have increased  in linear manner with the increased time of treatment. It reached 11.23% with replicate treatment of the dosage 1355 mg/kg.bw of the fungicide and 10.62% with the single treatment of the dosage 2493 mg/kg.bw. compared with 9.71 for negative control. The number of micronucleated polychromatic erythrocytes was significantly increased in linear manner with the replicate treatment of the dosage 1355 mg/kg.bw of the fungicide which reached 310.60 ± 1.36 and 243.60 ± 5.29 with the single dosage of 2493 mg/kg.bw of the fungicide compared with 2.80 ± 1.06 for negative control.

Treatment with Propamocarb-HCl induced damage in DNA of liver and bone-marrow cells. The value of OTM reached 58.40 ± 2.01 in bone-marrow cells with the single dosage of 2493 mg/kg.bw compared with 3.20 ± 0.66 for negative control. While it reached 61.80 ± 7.57 compared with 8.00 ± 1.38 for negative control in liver cells. The percentage of cells with damaged DNA reached 25.88%  in bone-marrow and 30.62% in liver.