Effect of add different carbon and nitrogen source on the pullulan polysaccharide production from fungus Aureobasidium pullulans

Authors

  • Mouhammed Basheer Ismaeel
  • Khalid Dham Ahmed
  • Zena Wajeeh Al.gader

DOI:

https://doi.org/10.25130/tjps.v21i5.1030

Abstract

Ten local isolates of Aureobasidium pullulans were isolated from different plant leaves. The ability of these isolates for pullulans production using production medium was investigated. The highest production of biomass and pullulans were achieved from isolates (A5, A2)with (11.93, 23.5)g/l respactivily.

The effect of eight carbon and eight nitrogen sources at final concentration (5%) on biomass and pullulan production from local isolates were tested. The results showed that glucose gave maximum production of polysaccharide from isolate (A7) with (15.5g/l).While the highest production of biomass were obtained when sucrose were used as a carbon source with (14.53)g/l from isolate (A5). Lactose and xylose failed to support polysaccharide and biomass production. Also Ammonium nitrate gave highest production of polysaccharide for all isolates tested were (22.9 g/l) from (A9). While the lowest production of polysaccharide and biomass were obtained when urea was used as a nitrogen source(1.15 ,0.28 g/l)from isolates (A6, A9) respectively .The highest production of biomass was obtained in case of using ammonium sulphat as a nitrogen source with (18.97 g/l) from isolate (A9).     

Downloads

Published

2023-02-06

How to Cite

Mouhammed Basheer Ismaeel, Khalid Dham Ahmed, & Zena Wajeeh Al.gader. (2023). Effect of add different carbon and nitrogen source on the pullulan polysaccharide production from fungus Aureobasidium pullulans. Tikrit Journal of Pure Science, 21(5), 67–71. https://doi.org/10.25130/tjps.v21i5.1030

Issue

Vol. 21 No. 5 (2016)

Section

Articles

License

Copyright (c) 2023 Tikrit Journal of Pure Science

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.