Determination of the effect of some biological products of Synechocystis pevalekii on some pathogenic bacteria isolated from wounds and urinary tract

Abstract

The sample were collected from different environments, growth and diagnosis of Cyanobacteria Species Synechocystis pevalekii was conducted in AL-Dour district / Salahaldeen governorate rocks having algae taken from the edge of the river, a water sample and mud sample from the river, and a soil sample from a planted land. The medium BG11 was used for its growth and measuring the daily increase of growth and chlorophyll these indicators were compared during their growth in four modified media of BG11 which were BG11+0.5g/L NaNo₃, BG11+0.006g/L (NH₄)₅[Fe(C₆H₄O₇)₂]2H₂S and BG11+0.1µ/L MoO₃ and the media BG11 was subjecte to all the mentioned additions, and the last was the medium BG11 was subjecte to all the mentioned additions the most effective in increasing daily growth and chlorophyll. Its bio- products were also extracted and the inhibitory efficacy of biological products of Synechocystis pevalekii was selected for species of bacteria isolated from wounds and urinary tract E.coli, Klebseilla pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis with the concentrations 10,000 , 7000 , 5000, 3000 , 2000 , 1000 µgm/ml . The concentration 10,000 µgm/ml showed a clear inhibitory effect E.coli 12mm, klebseilla pneumonia 5.5mm, Staphylococcus aureus 14 mm, Staphylococcus epidermidis 15.5mm whereas the concentration 1000 µgm/ml did not show any inhibitory effect for the growth of isolated bacteria under study. The other  concentrations showed varied effects. The active compound of the bio-product for the species of Synechocystis pevalekii was diagnosed by the device GC-Mass as a N-di-Alanylglycin. In addition the IR device was used and showed the active groups the alkanes and the amides and alkines and aldehydes and aminos.