Ag/ TiO2 core/shell NPs synthesized by laser ablation and its antibacterial activity

Abstract

As a consequence of the spread of infectious illnesses caused by numerous harmful bacteria and the development of antibiotic resistance, researchers and pharmaceutical firms are seeking for novel antibacterial medicines. Furthermore, due to their high surface area to volume ratio (SA:V) and unique physical and chemical characteristics, Nano scale materials are emerging as novel antibacterial agents in the contemporary context. In this study, we show how to prepare Ag/ TiO₂ NPs that are pure, stable, and high in concentration. By applying a pulsed laser (Nd:YAG) with different wavelengths of 1064 nm and a length of 532 nm resulting from the second harmonic generation using the KDP crystal, and with a wavelength of 355 nm resulting from the third harmonic generation using the nonlinear crystal KTP to a TiO₂ plate submerged in Ag nanoparticle suspensions previously produced with various energies of 200, 400, 600, 800, and 1000mJ, Ag/ TiO₂ NPs were created. The absorption peak for the sample obtained at 1000 mJ energy is greater than the absorption peak for the sample prepared at 200 mJ, which is attributable to increased nanoparticle concentration. At 440 nm, the remaining peaks are nearly fixed. The absorption rise when the laser wavelength was reduced from 1064 nm to 355 nm and the energy levels were increased from 200 to 1000 mJ, according to our findings. It was discovered that Ag/ TiO₂ has an effect on bacterial activity, with the inhibition area rising as the laser intensity rises. E.coli were shown to be more resistant to Ag/TiO₂than S.aureus.