Study of some Biochemical Parameters and Partial Purification of amylase from diabetic patients

Abstract

The aim of the study was to evaluate the level of amylase in the serum of diabetic patients compared to the level of healthy controls, in glucose, lipids, lipoproteins, thyroid hormones, electrolytes (calcium, potassium, and sodium) and transporter enzymes were also studied. (AST), (ALT),(ALP). the amylase enzyme was purified and the association between the enzyme and the previously mentioned variants was studied. it was studied amylase and other biochemical variables and it was found that there was a significant positive correlation when comparing the enzyme level with the variants (TG, VLDL, ALP) and there was no significant correlation. between enzyme activity and other biochemical variables. Where one peak was obtained in the fourth part of the purification, the degree of purification was 16.1; the result of the enzyme was 108.2% and the specific activity (0.189 ng/ mg, kinetic studies of the partially purified enzyme. The substrat concentration was 10 ng/mg, and the value of Michaelis Menten’s constant km (5.55 ng) and maximum velocity Vmax was  (0.98 ng/ml) the optimum temperature of the enzyme were reached (37 °C) while the optimum pH was (7.5).