Ability of L-Asparginase Produced from Locally E.coli Isolates on Negative Effect of UV Rays on Laboratory Rabbits

Abstract

This study was carried out to illustrate the effective of injected L-asparginase enzyme produced from E.coli isolate at 1 and 2 ml on natural and biochemical blood characterized in laboratory rabbits induced tumor through exposure to UV rays with a wavelength 256 ƞm for 28 days. Results indicated that the exposure to UV arrays from laboratory rabbits have caused an significantly (p< 0.05) reducing the total count of red blood corpuscular RBCs which became at 3.0×10⁶/mm³ and the hemoglobin concentration at 9.1 g/dl compared with the same value at animals control group which was at 6.5x10⁶/mm³ and 13.2 g/dl, respectively, as well as increased the total count of white blood cells WBCs and lymphocytes percentage deposited neutrophils percentage. Also, was caused significantly decline of total protein, albumin and globulin concentration in rabbits plasma and increase its liver enzymes activates for Aspartate amino transferase (AST), Alanine amino transferase (ALT), Alkaline phosphatase (ALP), which became at 52.3, 48.7 and 207 IU/ml, respectively, compared with the control group values at 32.0, 43.6, 141 IU/ml, respectively. The injection of an L-asparginase enzyme at 1 and 2 ml contains (72 IU/ml) due to inhibition of negative influence of UV rays altered in laboratory rabbits and improved the natural blood picture as red blood corpuscular, white blood cells, proteins parameters and liver enzymes activities significantly to close levels in control group animals. It can be concluded that bacterial isolates from E.coli species were as efficient in the L-asparginase enzyme production and the effectiveness of this enzyme were high in improving physiological parameters of laboratory rabbits exposed to UV rays.