Isolation and Identification of Bacteriodes Fragilis and Determination of the Synergest Effect of Some Antibiotics Used in Treatment Patients Infected with this Bacteria in Samarra Hosiptal

Abstract

The study dealts with isolating and identifying of B.fragilis from patients entered and visited the state hospital in samarra from 1/9/2013 to 1/5/2014. (150) samples were collected and the patients᾽ samples are distributed into (100) samples taken from the patients having diarrhea and (50) samples taken from the femal patients having complication followed the female surgical operations and the vaginal inflammation.

The results show that only (5) samples from all the diarrhea samples  develop positive bacteriological growth by B. fragilis  at ratio (5%). This study demonstrated that  the new-born infants and children who are less than 5 years are the most exposed to diarrhea caused by B. fragilis that the ratio of the infection is (7%) (11%) respectively, whereas the results for the smears taken from the female patients having complication followed the female surgical operations and the vaginal inflammation  show growth to B. fragilis in (3) samples and at ratio (6%), it is evident that the adult group of the women ranged between (14-20) years are the most exposed to the vaginal inflammations and the complication that followed the femal surgical operation.

It also tests the susceptibility of the bacterial isolates aginst (17) types of the antibiotic. The results  appear that the B. fragilis bacteria are extremely resistant to numerous antibiotics, so it appears a ratio of resistance to antibiotics (PRL), (AM), (P), (CTX), (AK), (TOB), (V), and (Eh). Their ratios are reaches (100%, 100%, 75%, 62%, 87%, 62%,  87%, 75%) respectively, whereas the B. fragilis are show a highly susceptibility aginst the antibiotics (C), (M), (DA), (AX), (IMP) and (G), so the ratio of their susceptibility  reaches to (100%) in each type of these antibiotics.

It also calculats the minimum inhibitory concentration (MIC) for the antibiotics: (M), (AM), (G), (CIP), (CTX) , (Eh) and (G). It is also tests the synergical activity for these antibiotics aginst the isolates  under-studied, which proves that the most effective synergies against B.fragilis is the synergy between (C) and (CTX) at ratio (60%) than the other isolates group. The synergy between (M) and( Rif) is shows an evident effect aginst the under-studied isolates with ratio (75%) from all the resistant isolates.