Isolation and Identification Bacteria Pseudomonas aeruginosa from adelt patients infected of otitis media and Curing of some Antibiotic Resistance and found content of G+C%

Authors

  • Intesar abduljabar shamkhe
  • Abdul Kareem Fattah Omar

DOI:

https://doi.org/10.25130/tjps.v21i5.1022

Abstract

Sixteen isolate of  Pseudomonas aeruginosa were collected from patients infected of otitis mediafor adelt people and diagnosed by biochemical teset and API20E these isolate tested for their resistance to Ampicillin,  Amoxillin, Chloramphenicol,  Trimethoprime, Tetracyclin, Cephotaxim, Amikacin, Gentamycin, Rifampicin and ciproflaxin. For the removal of the resistance  against the antibiotics in our isolates plasmid curing experiment was performed by using physical agent (elevated temperature). The results indicated that high temperature (47°C) was efficient to cure all the plasmids, and some isolates became sensitive to antibiotics. The extraction of chromosomal DNA was done to estimate the mol% of G+C of  DNA content to all  isolates the results the results showed that it was a taxonomic importance and it was at range (66.1- 67.3%).

Downloads

Published

2023-02-06

How to Cite

Intesar abduljabar shamkhe, & Abdul Kareem Fattah Omar. (2023). Isolation and Identification Bacteria Pseudomonas aeruginosa from adelt patients infected of otitis media and Curing of some Antibiotic Resistance and found content of G+C%. Tikrit Journal of Pure Science, 21(5), 19–23. https://doi.org/10.25130/tjps.v21i5.1022

Issue

Vol. 21 No. 5 (2016)

Section

Articles

License

Copyright (c) 2023 Tikrit Journal of Pure Science

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Tikrit Journal of Pure Science is licensed under the Creative Commons Attribution 4.0 International License, which allows users to copy, create extracts, abstracts, and new works from the article, alter and revise the article, and make commercial use of the article (including reuse and/or resale of the article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made, and the licensor is not represented as endorsing the use made of the work. The authors hold the copyright for their published work on the Tikrit J. Pure Sci. website, while Tikrit J. Pure Sci. is responsible for appreciate citation of their work, which is released under CC-BY-4.0, enabling the unrestricted use, distribution, and reproduction of an article in any medium, provided that the original work is properly cited.