Effect of aqueous extract of Asparagus officinalis on some Antioxidants in Rats Exposed to Oxidative Stress induced by Hydrogen peroxide

Abstract

The current study dealt with using  of hydrogen peroxide with 0.5% with drinking water to induce state of oxidative stress in male rats and estimate the ability of different concentrations of the aqueous extract of asparagus officinalis to reduce the effects of oxidative stress during periods 0,15,30 days by measuring the following parameters in serum: Glutathione(GSH), Malondialdehyde (MDA), the activity of super oxide dismutase enzyme (SOD) and the peroxynitrate radical (ONOO).

Fifty male rats with the age of 3-4 months and  weight of 300-400 gm were distributed into 5 groups: group (1): control group received drinking tap water, group (2): treated with H₂O₂ 0.5% in drinking water, group (3): treated with H₂O₂ and asparagus officinalis 5%, group (4): treated with H₂O₂ and asparagus officinalis 10%, group (5): treated with H₂O₂ and asparagus officinalis 20% . Hydrogen peroxide treatment led to a significant decrease (P <0.05) of the GSH and SOD activities  and increase of the MDA and ONOO levels in the serum during treatment periods compared with the control group. The results of using different concentrations of aqueous extract of asparagus on rats revealed similar effects but not identical in the protection from the oxidative stress induced by Hydrogen Peroxide , because its effect was affected on the level of GSH, SOD, MDA and ONOO levels in the blood serum in different experiment period.