A Study of Urinary Tract Infections Prevalence, Antibiotics Resistance, and Biofilm Formation Capability of the Bacterial Causal Agents

Authors

  • Safa Ali Naji
  • Halah Abdulkhaliq Awadh

DOI:

https://doi.org/10.25130/tjps.v27i6.765

Keywords:

UTI, Antibiotics, CRA, MTP, Biofilm.

Abstract

The aim of this study was to evaluate the incidence of Urinary Tract Infections (henceforth, UTI) in Salahadin governorate/ Iraq, identify the causative agents, their antibiotic sensitivity and their ability to form biofilms. Such studies are mandatory to determine the empirical therapy of such cases. A total of 650 urine samples were collected. Then, 250 samples were cultured, as suspected of having UTI, of which 193 samples (77.2 %) showed positive culture, while 57 samples (22.8 %) were negative cultures. Gram-positive bacteria were the predominant cause of UTI as represented by (66.7%), while Gram-negative species were found in (33.3%). Staphyloccus spp. bacteria were the predominant Gram-positive genus to isolate from UTI patients, while E. coli was the predominant species among Gram-negative bacteria. The isolates were tested for antibiotic susceptibility and biofilm formation. The most effective antibiotics tested on the Gram-positive isolates were Nitrofurantoin followed by Chloramphenicol (with resistance of 19% and 20%, respectively), whereas the least effective one was Azithromycin (with 64% resistance). As for the most effective antibiotic tested on the Gram-egative isolates, it was Amikacin (having only 8% resistance), while the least effective one was Erythromycin (with 98% resistance). Biofilm detection was performed by employing Congo red agar (CRA) method and microtiter plate (MTP) assay. The results showed that by using CRA method, about 81.3% of isolates were biofilm formers. While 96.7% of isolates were detected as biofilm formers by using MTP assay. Generally, biofilm formation was more predominant among Gram-positive isolates than Gram-negative ones.

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Published

2022-12-25

How to Cite

Safa Ali Naji, & Halah Abdulkhaliq Awadh. (2022). A Study of Urinary Tract Infections Prevalence, Antibiotics Resistance, and Biofilm Formation Capability of the Bacterial Causal Agents. Tikrit Journal of Pure Science, 27(6), 11–17. https://doi.org/10.25130/tjps.v27i6.765