Study of some parameters and Partial purification of prolidase from serum women preeclampsia
DOI:
https://doi.org/10.25130/tjps.v28i3.1423Keywords:
pre (preeclampsia), Enzyme prolidase, purification, Optimum condition.Abstract
This study was carried out by partial purification of prolidase from blood serum patients with preeclampsia by ion exchange The degree of purification enzyme (1. 85) fold, enzyme yield (8.82) and specific activity is (8.88) IU/mg .The kinetic studies of partially purified The enzyme technology demonstrated the optimal substrate concentration which was (97) mmol/L and Vmax (71.42 )(U/L),km(1.25) while optimal The optimum temperature was (37°C) and pH (9.2). molecular The weight of the partially purified enzyme was determined by a gel Electrophoresis method, in the presence of polyacrylamide gel and sodium Dodecyl Sulfate (SDS_PAGE) which showed that the approximate. The molecular weight was (54KD). We found a high level of Erythropoietin hormone in Pre-eclampsia patients which was a significant (P≤0.01) increase, (438.47 ) when she was in Control (251.70 ), the value of antidiuretic hormone in the patient was a significant (P≤0.05) increase (7.343) while in Control decrease value (2.155), the value of the ALD hormone in the patient was a significant decrease (P≤0.05) (288.7) and control was (143.6), Nitric Oxide in the patient was a significant (P≤0.05) (30.0) and control was increase (44.2) and vitamin D in the patient was a significant decrease (P≤0.05) (11.04) and control was(15.78) We conclude from the above results that there are some variables that rise with preeclampsia, such as the hormone ADH and the hormone EPO, but other variables such as ALD, vitamin D3 and nitric oxide decrease with increased pregnancy pressure, that is, with preeclampsia, in addition to that the main objective of the research is to purify the prolidease enzyme in the serum of women with preeclampsia and to know the duration of its effect This enzyme on pregnant women and linked it in another study with materials to find a new treatment
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