Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease

  • Amel Taha Yassein Al-Juraisy1
  • Ameera Aziz Mahmood Al-Juraisy1
  • Ameera Aziz Mahmood Al-Juraisy1
  • Nadia Ahmed saleh2

Abstract

The study includes partial purification of Lecithin: Cholesterol acyltransferase (LCAT) from the blood serum of a person suffering from atherosclerosis. Several techniques were applied including ammonium sulphate precipitation, dialysis, ion exchange chromatography and electrophoresis. It was found that LCAT has one isoenzyme and the highest activity was (1073.46 × 10-3) unit/ml and a molecular weight of 62 KDa. The study  also deals with characterizing of LCAT. It was found that the optimum pH and temp. of the enzyme were 7 and 35ºC.


 


 http://dx.doi.org/10.25130/tjps.25.2020.027

Published
Mar 17, 2020
How to Cite
AL-JURAISY1, Amel Taha Yassein et al. Biochemical and Kinetic Study for the partially purified Lecithin: Cholesterol acyltransferase from serum cardiovascular disease. Tikrit Journal of Pure Science, [S.l.], v. 25, n. 2, p. 31-37, mar. 2020. ISSN 2415-1726. Available at: <http://tjps.tu.edu.iq/index.php/j/article/view/955>. Date accessed: 02 apr. 2020. doi: http://dx.doi.org/10.25130/j.v25i2.955.
Section
Articles

Most read articles by the same author(s)