Some haematological and biochemical parameters assessments in sheep infection by Haemonchus contortus

Abstract

The study was conducted on (100) sheep naturally infested with Haemonchus  contortus, (1-3 years old), in Tikrit city, from ist January 2014 to 31th May 2014. Affected sheep exhibited clinical signs included, emaciation, weakness, pale mucous membranes, rough, easily detached and lusterless coat. Ten  ml of blood samples were collected from the jugular vein of all the sheep's  using disposable syringes, then take 2.5 ml from blood sample was used to determine CBC the Red blood cells (RBC), white blood cells (WBC) , Haemoglobin estimation (Hb), Packed cells Volume (PCV). The study showed reduction in RBC, hemoglobin, hematocrit and neutrophils (4.7±0.68), (6±0.74), (18±0.19), and (20±0.73) respectively. And reveled leukocytosis; Lymphocyte, eosinophil's and monocyte (11.6± 0.41), (65± 0.51), (10± 0.64) and (5± 0.29) respectively in infected sheep. and the remind samples were centrifuged at 3000 rpm for 10 minute and serum was separated and stored at -20 °c until used for measure the biochemical change by use spectrophotometer. The results showed a significant decrease (P < 0.05) in the rates of total protein concentration, albumin , iron, and zinc (5.02± 0.59 g/dl), (1.7 ± 0.45g/dl), (89.3 ± 12.41 µg/dl), (42.37± 16.22 µg/dl)  respectively in the serum of infected sheep according the compared with normal sheep.