Estimation of 5`-nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes

Abstract

This study was performed on (60) saliva specimens, including (30) diabetic patients in addition (30) healthy saliva specimens were taken from healthy subjects as control group. The results showed a significant increase in the activity of saliva 5`-nucleotidase (5`-NT) from diabetic patients compared to healthy subjects.

This study included a partial purification of 5`-NT as well, using (35%) (NH₄)₂SO₄   for precipitation and gel filteration by (sephadex G-75 ) which gave single peak. Then 5-NT was partially  also purified by using Ion exchange chromatography ( DEAE-cellulos A-50), in which three isoenzymes were isolated with different degree of purity.