Isolation and partial Purification and for amylase from diabetic patients and some kinetic properties
Abstract
Amylase is an enzyme which is secreted from pancreas, and salivary glands (α-Amylase EC3.2.1.1), there is a small amount of this enzyme presents in the blood ranging between 100–300 I.U/L, when this percentage in the blood would increase,it means that the above organs are ill. The study included apartial purification of this enzyme from the blood of diabetic patients using gel, filtration, dialysis, and sephadex G100 gel, the single beak was obtained in the fourth part of purification and the degree of purification reached 16.1 with an enzymatic outcome of 108.2% and specific activity was 0.189ng/mg. The kinetic study of the purified enzyme appeared an optimum concentration of the substrate of 10 ng / mg,the Km was 5.55 ng , maximum acivity Vmax, was 0.98ng / ml,the optimum temp. was (37C°) and the opt. pH was 7.5.
