Isolation and partial Purification and for amylase from diabetic patients and some kinetic properties

  • Wasan Abd alhassan Hamad
  • Firas Taher Maher

Abstract

Amylase is an enzyme which is  secreted from  pancreas, and salivary glands (α-Amylase EC3.2.1.1), there is a small amount of this enzyme  presents in the blood ranging between 100–300 I.U/L, when this percentage in the blood would increase,it means that the above organs are ill. The study included  apartial purification of this enzyme from the blood  of diabetic patients using  gel, filtration, dialysis, and sephadex G100 gel, the single beak was obtained in the fourth part of purification and the degree of purification reached 16.1 with an enzymatic outcome of 108.2% and specific activity was 0.189ng/mg. The kinetic study of the purified enzyme appeared an optimum concentration of the substrate of 10 ng / mg,the Km was 5.55 ng , maximum acivity Vmax, was 0.98ng / ml,the optimum temp.  was (37C°) and the opt. pH was 7.5.


 


http://dx.doi.org/10.25130/tjps.25.2020.110

Published
Dec 23, 2020
How to Cite
HAMAD, Wasan Abd alhassan; MAHER, Firas Taher. Isolation and partial Purification and for amylase from diabetic patients and some kinetic properties. Tikrit Journal of Pure Science, [S.l.], v. 25, n. 6, p. 54-58, dec. 2020. ISSN 2415-1726. Available at: <http://tjps.tu.edu.iq/index.php/j/article/view/1068>. Date accessed: 08 dec. 2021. doi: http://dx.doi.org/10.25130/j.v25i6.1068.
Section
Articles