Isolation of Trypsin from Serum of pancreatic Cancer Patients and determination some biochemical parameters

  • Zena A. Mohammad
  • Layla A. Mustafa

Abstract

The research included isolation and purification of Trypsin from serum of patient with pancreatic cancer, and estimation its molecular weight by using the two techniques of gel filtration (using sephadex G-50) and electrophoresis (using SDS). Therefore; it was noted that the proteinous peak  isolated from ammonium sulfate solution is saturated (65%) of the serum by using the two techniques gel filtration and electrophoresis had apparent molecular weight (27000) Dalton. The study included (40) patients with pancreas cancer their ages ranged between (30-70) years who attended Al-Jumhuri and Atomic Medicine Hospitals in Mosul; the laboratory examination included estimation levels of: Trypsin, Gamma Glutamyl transferase, Amylase, Paraoxonase  ,Glutathione, Sialic acid and Ceruloplasmin. Results showed that level of Trypsin and Amylase in the serum decreased significantly in  patients with pancreatic cancer in comparison with the control group, but levels of Gamma Glutamyl transferase, paraoxonase, Glutathione, Sialic acid and Ceruloplasmin increased significantly in patients with pancreatic cancer in comparison with the control group.


http://dx.doi.org/10.25130/tjps.23.2018.085


 

Published
Aug 6, 2018
How to Cite
MOHAMMAD, Zena A.; MUSTAFA, Layla A.. Isolation of Trypsin from Serum of pancreatic Cancer Patients and determination some biochemical parameters. Tikrit Journal of Pure Science, [S.l.], v. 23, n. 6, p. 31-35, aug. 2018. ISSN 2415-1726. Available at: <http://tjps.tu.edu.iq/index.php/j/article/view/105>. Date accessed: 17 nov. 2019.
Citation Formats
Issue
Vol 23 No 6 (2018)
Section
Articles